Description
Aviva Systems Biology Pcsk1n ELISA Kit (Rat) (OKEH06477) is based on a competitive enzyme immunoassay technique. The microtiter well-plate in this kit has been pre-coated with an anti-Pcsk1n antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Pcsk1n and incubated. The Pcsk1n found in the sample or standards competes with the biotinylated Pcsk1n for limited binding sites on the immobilized anti-Pcsk1n antibody. Excess unbound biotinylated Pcsk1n and sample or standard Pcsk1n is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Pcsk1n captured in the well and inversely proportional to the amount of Pcsk1n which was contained in the sample or standard